Intestinal Necrosis
Intestinal Necrosis - Related Videos
02:12
Muscle Necrosis Assay in C. elegans Induced by a Pathogenic Aeromonas Strain
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2026
Source: Chen, Y. et. al., Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model. J. Vis. Exp. (2018)This video demonstrates a muscle necrosis assay in C. elegans (Caenorhabditis elegans) using pathogenic Aeromonas strains. By feeding green fluorescent protein (GFP)-expressing worms on Aeromonas-seeded plates, the assay models bacterial infection and reveals tissue damage through fluorescence imaging. The progressive loss of muscle structure and GFP...
01:16
Necrosis
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2023
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”. Morphological Manifestations of Necrosis Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
01:16
Cellular Injury IV: Necrosis
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2026
Necrosis is a form of irreversible cell death caused by severe injury such as ischemia, toxins, or trauma. Unlike programmed cell death, it is an uncontrolled, pathological process that typically provokes inflammation in surrounding tissues.Pathophysiologic ChangesNecrosis begins when cells sustain critical damage, leading to swelling of organelles, particularly mitochondria, and rapid ATP depletion. As energy levels decline, membrane ion pumps fail, leading to calcium influx and eventually,...
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
FAQs
When oxygen delivery falls, intestinal cells cannot sustain normal energy production. This disruption weakens cellular function and damages the intestinal barrier, which normally limits harmful effects beyond the bowel. As injury continues, tissue may break down rather than recover. This mechanism explains why prompt recognition is important before local damage progresses to broader complications.
Progressive tissue breakdown can compromise the bowel wall and lead to perforation, allowing a previously localized process to become more extensive. Infection and systemic inflammation may then develop, affecting the patient beyond the intestine. The possibility of this progression makes intestinal necrosis a time-sensitive medical problem rather than an isolated structural abnormality.
The key distinction is whether the intestinal tissue can recover after its blood supply is restored or has already progressed to nonviable tissue. Clinical and imaging features support this assessment, although the overview does not specify individual findings. Making the distinction matters because treatment may focus on restoring perfusion or removing bowel that cannot recover.
Clinical assessment provides information about the patient’s condition, while imaging helps evaluate the bowel and identify features associated with ischemia or advanced injury. Together, these sources support urgent evaluation and help distinguish potentially reversible ischemia from irreversible damage. Their combined use informs decisions about whether perfusion restoration or removal of nonviable bowel is needed.
The initial approach is urgent assessment aimed at recognizing ischemic or necrotic injury, evaluating whether the damage remains reversible, and identifying complications such as perforation, infection, or systemic inflammation. Clinicians then use the assessment to guide treatment. The central goal is to limit progression by addressing impaired perfusion or nonviable tissue.
Restoring perfusion is relevant when impaired blood supply remains the central problem and the affected tissue may still recover. Removing bowel is considered when tissue has become nonviable and cannot be preserved. These treatment paths reflect the distinction between reversible ischemia and irreversible injury, with both intended to limit complications and improve outcomes.