Peritoneal Macrophage Isolation
Peritoneal Macrophage Isolation - Related Videos
08:21
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Cited by 78 •
2015
We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.
05:36
Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages
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2025
This video demonstrates a procedure for analyzing peritoneal macrophage-mediated efferocytosis of apoptotic mouse thymocytes by fluorescence microscopy.
03:14
Assessing the Survival of a Bacterial Pathogen in Mouse Peritoneal Macrophages
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2026
Source: Zhang, C. et al. Development and Assessment of Intracellular Infection Models for Staphylococcus aureus. J. Vis. Exp. (2025)This video demonstrates the quantification of intracellular survival of a methicillin-resistant bacterial pathogen in mouse peritoneal macrophages. It outlines the steps involved in infection, extracellular bacterial clearance, host cell lysis, and colony enumeration to assess bacterial survival within host macrophages.
04:32
Isolation of Mouse Peritoneal Cavity Cells
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Cited by 445 •
2010
The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.
05:05
In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
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2025
This video demonstrates the activation of a mouse's peritoneal macrophages using intravenous immunoglobulin and bacterial lipopolysaccharide. The co-stimulation activates the peritoneal macrophages into anti-inflammatory macrophages possessing immune regulatory activity.
FAQs
After peritoneal lavage and centrifugation, the recovered cell mixture is placed under culture conditions that support macrophage adherence. Macrophages attach to the culture surface, whereas nonadherent cells can be separated from them. This step enriches the macrophage population for subsequent examination of morphology, viability, surface markers, phagocytosis, cytokine production, or responses to experimental stimuli.
Peritoneal lavage retrieves resident immune cells from the peritoneal cavity into a recoverable cell suspension. Centrifugation then concentrates those cells so they can be placed in culture and processed further. Together, these steps connect cell recovery with enrichment by adherence, creating a practical preparation for studying macrophage behavior outside the whole-animal setting.
Culture conditions and the selected experimental stimulus strongly shape the responses that can be measured. Conditions that support macrophage adherence enable separation from nonadherent cells, while inflammatory stimuli or drugs allow investigators to examine functional changes. The resulting observations may include altered cytokine production, phagocytosis, morphology, viability, or surface-marker profiles.
A typical workflow begins with peritoneal lavage to collect resident immune cells, followed by centrifugation to concentrate the recovered population. The cells are then placed under culture conditions that promote macrophage adherence. Nonadherent cells are separated from the attached population, after which the macrophages can undergo morphological, viability, marker, functional, or treatment-response analyses.
The isolated cells support several complementary readouts. Morphology and viability describe cellular condition, while surface markers help characterize the population. Functional assays can examine phagocytosis and cytokine production, and treatment experiments can test responses to drugs or inflammatory stimuli. Using multiple readouts helps connect macrophage state with innate immune or inflammatory activity.
This preparation provides an accessible ex vivo model for investigating host defense, infection, tissue injury, and immune-mediated disorders. Because the cells can be challenged with drugs or inflammatory stimuli and then analyzed directly, investigators can examine macrophage responses without the full complexity of a whole-animal experiment. The approach therefore supports focused studies of disease-related immune mechanisms.