Porcine Liver Transplantation
Porcine Liver Transplantation - Related Videos
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
05:25
Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation
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2024
This protocol outlines the methodology of establishing a porcine model utilizing variable temperature-controlled Machine Perfusion (MP) for the preservation of the donor liver, followed by orthotopic liver transplantation (OLTx). It aims to promote the success rate of OLTx using donor donation after circulatory death (DCD) liver and establish a stable model.
18:25
Orthotopic Liver Transplantation in Rats
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Cited by 31 •
2012
We present an easy-to-establish revision of the classical two-cuff technique for orthotopic liver transplantation in rat.
08:37
Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels
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2025
Here, we present a protocol to establish a long-term liver cold storage and transplantation model in Daurian ground squirrel (DGS), which has been technically challenging in standard rodent models.
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
FAQs
The recipient’s immune system can recognize pig liver cells and tissues as foreign, creating a major barrier to sustained function. This response may contribute to rejection and inflammation, so studies examine how immune recognition affects graft performance and how immunosuppressive therapy can limit damaging reactions. Controlling this interaction is essential when assessing whether porcine tissue can support human physiology.
Blood-group compatibility and coagulation are separate but connected safety concerns. Species-specific differences may interfere with blood handling and clotting after the liver is connected to the recipient’s circulation. These factors can influence inflammation, graft performance, and postoperative stability, making them important variables in experimental design and in evaluating whether a porcine liver can function effectively in a human setting.
Genetic modification of the donor animal is investigated to reduce biological incompatibilities between pig tissue and the human recipient. Immunosuppressive therapy addresses the recipient’s immune response after transplantation. Used as complementary strategies, they aim to improve graft acceptance while preserving liver activity. Research must determine whether these interventions limit rejection and inflammation without undermining the intended support of liver function.
Careful control of surgical and postoperative conditions is necessary because graft performance depends on more than the liver itself. Investigations monitor the recipient’s response, blood handling, coagulation, inflammation, and signs of infection while assessing liver activity. This controlled approach helps distinguish technical or postoperative complications from limitations caused by species differences or inadequate biological function.
The approach is being investigated as a temporary bridge for people with acute liver failure or for situations in which a suitable human donor organ is unavailable. The goal is to provide enough liver support to maintain essential function during a critical interval. Its value therefore depends on whether the porcine liver can sustain the recipient while longer-term treatment or transplantation options are pursued.
Studies assess whether the porcine liver provides metabolic detoxification, protein synthesis, and other essential functions required for physiological support. They also evaluate rejection, inflammation, infection, coagulation, and overall graft performance. Together, these outcomes show not only whether the tissue works, but also whether its benefits are sufficient to justify further development of temporary support systems or future clinical transplantation strategies.