Retina Pinning
Retina Pinning - Related Videos
07:53
Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina
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2024
This work presents an alternative flatmount retina preparation in which the removal of photoreceptor cell bodies enables faster antibody diffusion and improved patch pipette access to inner retinal neurons for immunohistochemistry, in situ hybridization, and electrophysiology experiments.
10:12
Organotypic Culture of Adult Rabbit Retina
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Cited by 8 •
2007
This article demonstrates the dissection and incubation of rabbit retina and particle-mediated gene transfer of plasmids encoding GFP or a variety of subcellular markers into retinal ganglion cells.
01:21
Euler's Formula for Pin-Ended Columns
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2024
In structural engineering, the stability of columns under compressive axial loads is a critical consideration, described as buckling. A typical example involves a column PQ, which is pin-connected at both ends and subjected to a centric axial load F applied at one end, with a reaction force of F' = -F at the other end. Here, it is crucial to understand that when an applied load exceeds the critical load, buckling occurs as the system becomes unstable. To calculate the critical load, envision...
06:15
In Ovo Electroporation in Embryonic Chick Retina
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Cited by 10 •
2012
The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.
05:53
In vivo Electroporation of Developing Mouse Retina
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Cited by 33 •
2011
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
FAQs
Fine pins provide mechanical stabilization by anchoring the isolated tissue to a support surface. This limits movement and helps maintain a consistent orientation while the retina remains accessible for inspection of its layers and anatomical regions. That controlled geometry is important when researchers need to examine comparable areas across specimens.
A flattened specimen offers researchers a more uniform field for viewing retinal layers and selected anatomical regions. By reducing positional variation between preparations, the technique supports more consistent examination of cellular organization and disease-related changes. This matters in comparative experiments, where differences should reflect tissue condition rather than inconsistent specimen orientation.
Because the tissue remains secured during handling, researchers can examine comparable regions across specimens using a reproducible preparation. Retina Pinning therefore contributes to controlled comparisons of cellular organization and retinal responses. It is especially useful when experiments assess injury, degeneration, or other ocular disorder models.
The workflow begins with an isolated retina, which is positioned on a support surface and secured with fine pins. Researchers then keep the tissue flattened and oriented while carrying out the planned examination or experiment. This arrangement provides access to retinal layers and anatomical regions for subsequent preparation, imaging, staining, or physiological assessment.
After stabilization, the preparation can support whole-mount work, microscopy, immunohistochemistry, or ex vivo physiological studies. Each use benefits from access to retinal layers and anatomical regions without the specimen shifting during handling. Consequently, researchers can examine structure, characterize cellular organization, or assess retinal responses within a controlled tissue preparation.
Within medicine-focused research, pinned retinas can be used to study cellular organization and disease-related changes in models of injury, degeneration, and other ocular disorders. The controlled preparation also supports assessment of retinal responses during ex vivo experiments. Its main value is creating specimens that can be examined and compared under consistent spatial conditions.