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In vivo Electroporation of Developing Mouse Retina
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JoVE Journal Neuroscience
In vivo Electroporation of Developing Mouse Retina

In vivo Electroporation of Developing Mouse Retina

DOI:
10.3791/2847-v

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05:53 min

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June 24, 2011

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1Solomon H. Snyder Department of Neuroscience,Johns Hopkins School of Medicine, 2Department of Neurology,Johns Hopkins School of Medicine, 3Department of Ophthalmology,Johns Hopkins School of Medicine, 4Center for High-Throughput Biology,Johns Hopkins School of Medicine, 5Institute for Cell Engineering,Johns Hopkins School of Medicine

Chapters

  • 00:05Title
  • 01:52Concentration and Subretinal Injection of Plasmid DNA
  • 03:46Electroporation
  • 03:46Neonatal Mouse Retinas Electroporated with pCAG-EGFP Over Developmental Time
  • 05:15Conclusion

Summary

Automatic Translation

Automatic Translation

A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.

Tags

In Vivo ElectroporationDeveloping Mouse RetinaGene TargetingLoss Of Function KnockoutsGain Of Function ExperimentCell Fate SpecificationTerminal DifferentiationDNA PlasmidsNeonatal Mouse RetinaElectrical ImpulsesPlasma Membrane PermeabilityGene TransferHydrophilic Plasma Membrane Pores

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