Splenic Tumor Injection
Splenic Tumor Injection - Related Videos
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
08:02
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
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Cited by 4 •
2021
This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings.
03:42
Modeling Brain Tumor Metastasis via Internal Carotid Artery Injection of Cancer Cells
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2025
Source: Lim, M., et al. Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells. J. Vis. Exp. (2022)In this video, an anesthetized mouse undergoes a surgical procedure in which cancer cells are injected into the common carotid artery (CCA) to facilitate targeted delivery to the brain. The procedure involves precise suturing and manipulation of the carotid arteries to direct the injected cells toward the internal carotid artery (ICA), promoting tumor formation in the brain...
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
04:08
Intracecal Tumor Cell Injection: A Technique to Deliver Cancer Cells for Establishing Orthotopic Colorectal Cancer Mouse Model
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2023
The video describes the technique for intracecal injection of cancerous cells to develop an orthotopic mouse model with highly uniform tumors. Therefore, the orthotopic mouse model allows realistic simulation of metastasis, providing a valuable tool to study the phenotype changes during tumor growth and dissemination.
FAQs
Tumor cells established in splenic tissue can enter the portal circulation, creating an experimental route for studying dissemination to the liver and other sites. This feature connects local tumor growth with patterns of spread beyond the injection site. Consequently, the model supports investigation of metastatic progression and interactions between tumor cells and organs involved in disease development.
The technique can begin with either tumor cells or tumor tissue, allowing researchers to introduce the experimental tumor material into the spleen under controlled conditions. Both formats support investigation of subsequent growth and dissemination in vivo. The selected material therefore becomes an important part of experimental design when comparing disease mechanisms or evaluating anticancer treatment responses.
Growth within the spleen and dissemination to the liver or other sites allow researchers to examine cancer as an interaction between tumor material and host organs. This perspective extends analysis beyond tumor expansion alone. Findings can help relate metastatic progression to organ-specific environments and support studies of how disease develops across multiple anatomical locations.
Because the approach produces an in vivo model of tumor growth and dissemination under controlled experimental conditions, it can serve as a platform for assessing responses to anticancer treatments. Researchers can relate treatment effects to disease progression, metastatic spread, or tumor–organ interactions. Its reproducibility also helps support comparisons across treatment strategies when experimental conditions remain consistent.
Injection accuracy, animal welfare, and experimental consistency are central procedural considerations. Accurate delivery helps ensure that the intended splenic site receives the tumor cells or tissue, while welfare practices address the ethical requirements of in vivo research. Consistent handling and study conditions improve reproducibility, making differences in tumor growth, dissemination, or treatment response easier to interpret.
Researchers may choose this model when they need to study cancer progression in vivo, particularly growth within splenic tissue and dissemination through the portal circulation. It is relevant for investigating liver or other-site involvement, tumor–organ interactions, and therapeutic responses. The technique therefore connects mechanistic cancer research with preclinical evaluation of potential treatment strategies.