Micro Pipette
Micro Pipette - Related Videos
10:36
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This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
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The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
05:02
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05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Cited by 13 •
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This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
FAQs
Pressing the spring-loaded plunger moves a piston through a calibrated chamber and changes the pressure inside the system. Releasing the plunger creates the pressure change that draws liquid into the disposable tip, while pressing it again expels the selected volume. This sequence links plunger movement to controlled liquid transfer during sample preparation.
The calibrated chamber provides a defined relationship between piston movement and the liquid volume selected for transfer. Because neuroscience experiments often require accurately prepared buffers, diluted reagents, or cell suspensions, this calibration supports consistent concentrations. Reliable chamber calibration therefore helps reduce variation between preparations and strengthens the reproducibility of downstream molecular, cellular, and electrophysiological studies.
Inconsistent pipetting can alter the concentration of prepared solutions, increase sample loss, or produce variation between experimental preparations. Those changes may affect solutions used with neurons, synapses, or brain tissue and can weaken comparisons across samples. Consistent operation is therefore important when preparing materials for molecular, cellular, and electrophysiological research.
A typical sequence uses a disposable tip, sets the required volume, and operates the spring-loaded plunger to control aspiration and dispensing. Releasing the plunger draws the liquid into the tip through a pressure change, and pressing it expels the selected amount. Applying this sequence consistently supports accurate transfer of buffers, reagents, dilutions, or cell suspensions.
Researchers can use this instrument to prepare buffers, dilute reagents, and transfer cell suspensions in neuroscience workflows. These materials may support experiments involving neurons, synapses, and brain tissue. Accurate small-volume handling helps maintain the intended composition of solutions and limits sample loss during preparation, providing more consistent inputs for subsequent molecular or cellular studies.
Electrophysiological studies may require solutions whose concentrations are prepared consistently before use. Micro pipette handling supports this preparation by transferring selected volumes and helping reduce variation in buffers, diluted reagents, or other solutions associated with neuronal experiments. More consistent preparation improves the reliability and reproducibility of studies examining electrical activity and related cellular processes.