Mobile Fraction
Mobile Fraction - Related Videos
10:09
Fractional Distillation
0 Views •
2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
06:57
Electrophoretic Mobility Shift Assay (EMSA)
0 Views •
2023
The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...
01:26
Mobile Marketing
0 Views •
2024
As consumers increasingly rely on smartphones and other mobile devices for browsing, communication, and shopping, mobile marketing has become essential in today's digital world. Brands must adjust their strategies to engage users where they spend most of their time, which is on mobile screens. Understanding mobile user behavior is crucial for successful mobile marketing. Mobile users typically interact with their devices briefly and frequently throughout the day. They prefer content that is...
08:40
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
0 Views •
Cited by 2 •
2022
Ion mobility spectrometry (IMS) is an interesting complement to mass spectrometry for the characterization of biomolecules, notably because it is sensitive to isomerism. This protocol describes a tandem IMS (IMS/IMS) experiment, which allows the isolation of a molecule and the generation of the mobility profiles of its fragments.
07:57
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
0 Views •
Cited by 17 •
2009
The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
FAQs
FRAP recovery can arise from two related processes: unbleached molecules may diffuse into the bleached region, or molecules may exchange between bound and unbound states. Consequently, the measured mobile fraction reflects the portion capable of movement or exchange during the observation, while also indicating how much of the population remains immobilized or tightly retained.
Receptor anchoring and cytoskeletal interactions can reduce the mobile fraction by retaining molecules at particular neuronal locations. Signaling activity may alter that balance, changing how freely molecules redistribute or exchange. Examining these influences helps connect molecular mobility with the organization of synapses and the regulation of neuronal communication.
Comparing mobile fractions is informative because identical molecular populations may show different retention in neuronal membranes, synapses, or intracellular compartments. A difference suggests that local anchoring, cytoskeletal association, or signaling-related conditions influence mobility. The comparison therefore links a fluorescence measurement to changes in molecular organization rather than treating recovery as an isolated optical signal.
To estimate the mobile fraction with FRAP, researchers fluorescently label the molecules, select a region, and briefly photobleach that area. They then measure fluorescence recovery as unbleached molecules enter or exchange within the region. The resulting recovery is interpreted in relation to the bleached population to estimate mobile and retained portions.
A higher mobile fraction indicates that a larger share of the labeled population can move or exchange during the measurement. A lower fraction indicates greater immobilization or tight binding. In neuronal samples, this contrast can reveal differences in receptor retention or molecular organization across membranes, synapses, and intracellular compartments.
In synaptic research, mobile fraction measurements help assess whether receptors and other labeled molecules remain stably retained or redistribute within neuronal structures. Relating those measurements to anchoring, cytoskeletal interactions, or signaling activity can clarify how synaptic organization changes and how such organization may influence neuronal communication.