Superficial Dorsal Horn
Superficial Dorsal Horn - Related Videos
09:48
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration
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Cited by 9 •
2017
This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.
07:12
Studying the Coding Profiles of Somatic Stimulation on Cardiac-locked Neuronal Responses in the Rat Spinal Dorsal Horn
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2025
We describe a protocol for spinal multichannel extracellular recording alongside cardiac function recording and analyzing the cardiac-locked spinal dorsal horn neurons. This method offers a temporally synchronized framework for studying spinal mechanisms underlying thoracic visceral functional changes induced by acupuncture.
10:49
Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury
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Cited by 30 •
2011
Neural precursor transplantation is a promising strategy for protecting and/or replacing lost/dysfunctional cervical phrenic motor neurons in spinal cord injury (SCI) and the motor neuron disorder, amyotrophic laterals sclerosis (ALS). We provide a protocol for cell delivery to cervical spinal cord ventral horn in rodent models of ALS and SCI.
04:38
Modeling Sensory Axon Regeneration in a Rat Using Dorsal Root Ganglion Injection and Dorsal Root Crush Injury
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2025
Source: Cheah, M., et al. Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration. J. Vis. Exp. (2017).This video demonstrates a step-by-step procedure for injecting a viral suspension into the dorsal root ganglia (DRGs) and inducing a controlled axonal injury via a dorsal root crush in an anesthetized rat. The protocol involves exposing the DRGs, delivering a growth-promoting viral vector to facilitate axonal regeneration, and severing axonal...
09:40
Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats
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Cited by 11 •
2016
Non-invasive assessment of endothelial function in humans can be determined by the flow-mediated dilation technique. Although thousands of studies have used this technique, no study has performed this technique non-invasively in rats. The following article describes non-invasive measurement of flow-mediated dilation in the brachial and superficial femoral arteries of rats.
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Laminae I and II organize much of the superficial dorsal horn’s initial sensory processing. Primary afferent fibers deliver peripheral information to these laminae, where local excitatory and inhibitory interneurons modify the incoming activity. This arrangement allows signals associated with pain, temperature, itch, and other protective sensations to be processed before information is passed toward projection neurons and ascending pathways.
Excitatory and inhibitory interneurons provide opposing forms of local control over incoming sensory activity. Their interaction helps transform primary afferent input rather than simply relay it unchanged. This local processing contributes to how peripheral stimuli acquire spinal sensory representations and how protective sensations are modulated before signals engage projection neurons and longer-range ascending pathways.
Projection neurons represent a later stage in the superficial dorsal horn’s processing sequence. After primary afferent input has been shaped by local excitatory and inhibitory interneurons, projection neurons provide a route toward ascending pathways. Studying this transition helps researchers connect local spinal circuitry with the broader transmission of pain, temperature, itch, and related sensory information.
Signal transformation explains how activity arriving from the periphery becomes a structured sensory representation within the spinal cord. The superficial dorsal horn does not merely receive incoming signals; its local circuitry modifies them before onward transmission. This principle is important for understanding why pain, temperature, itch, and other protective sensations depend on spinal processing and modulation.
Research can examine how primary afferent input is organized, how local excitatory and inhibitory interneurons alter that input, and how processed activity reaches projection neurons and ascending pathways. These questions connect peripheral stimulation with spinal sensory representations. They also provide a framework for investigating the circuitry underlying pain, temperature, itch, and other protective sensations.
Its circuitry offers a spinal location for examining how sensory processing and modulation may relate to abnormal sensitivity. By studying the interactions among incoming afferent signals, local interneurons, and projection neurons, researchers can investigate mechanisms associated with sensory hypersensitivity and chronic pain. The region therefore links basic circuit organization with clinically relevant changes in protective sensation.
The superficial dorsal horn contains several identifiable stages of sensory processing, including primary afferent input, local excitatory and inhibitory control, and transmission through projection neurons. This organization helps researchers identify where modulation occurs within pain and related sensory pathways. Understanding these circuit mechanisms can support investigations of potential targeted therapies for chronic pain and sensory hypersensitivity.