Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at <em>Drosophila</em> Neuromuscular Junctions

Nicholas J. Hornstein; Stefan R. Pulver; Leslie C. Griffith

doi:10.3791/1133

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Biology

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Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions

Channelrhodopsin2介导的突触电位刺激果蝇神经肌肉接头

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09:12 min

March 16, 2009

DOI: 10.3791/1133-v

Nicholas J. Hornstein¹, Stefan R. Pulver¹, Leslie C. Griffith¹

¹Department of Biology,Brandeis

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此过程使用蓝色光激活藻通道和细胞特异性基因表达的工具，以唤起光脉冲在神经肌肉接头（NMJ）突触潜力

这个过程从生长果蝇开始。第三，在含有所有反式视黄醇的食物上具有适当基因型的龄蛆。每只蛆虫的头和尾都被钉住了。

然后是 FLA flat。然后切断大脑和后神经以停止有节奏的运动活动。接下来，调整带有控制箱的蓝色 LED 光源并专注于准备工作。

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