Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

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被引用 45 次

07:03 分钟

2009年7月2日

10.3791/1266-v

2009年7月2日

34.8K 次观看

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

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D I 70

Chapters in this video

0:04

Title

0:31

Introduction

1:01

Preparing Enzyme Solutions and Buffers

1:50

Dissociating the Lung Tissue

4:10

Filtration

5:35

Representative Results

6:07

Conclusion

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