Floral-dip Transformation of <em>Arabidopsis thaliana</em> to Examine <em>pTSO2::&beta;-glucuronidase</em> Reporter Gene Expression

Chloe Mara; Boyana Grigorova; Zhongchi Liu

doi:10.3791/1952

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

花香浸改造拟南芥检查 pTSO2:β-葡萄糖醛酸酶报告基因的表达

Full Text

37,226 Views

10:24 min

June 11, 2010

DOI: 10.3791/1952-v

Chloe Mara¹, Boyana Grigorova¹, Zhongchi Liu¹

¹Department of Cell Biology and Molecular Genetics,University of Maryland College Park

Please note that some of the translations on this page are AI generated. Click here for the English version.

本文介绍了花卉浸方法

花浸渍用于转化具有报告基因构建体的拟南芥植物。然后检查所得转基因植物的报告基因表达。首先，将幼嫩开花的拟南芥植物浸入含有报告基因构建体的农杆菌到以弗所的溶液中。

接下来，植物恢复到正常的生长条件，直到它们产生种子，其中一小部分将被外源基因转化。该程序的第三步是从植物中收集种子并将其播种在含有抗生素的选择培养基上。最后，检查在含有抗生素的选择培养基上生长的幼苗。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

细胞生物学第40期花香浸改造农杆菌介导法 β-葡萄糖醛酸酶（GUS）的记者细胞周期核苷酸还原酶（RNR）拟南芥