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JoVE Encyclopedia of Experiments
Cancer Research
兔导管内注射:将目标溶液局部输送到兔乳腺中
兔导管内注射:将目标溶液局部输送到兔乳腺中
Encyclopedia of Experiments
Cancer Research
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Encyclopedia of Experiments Cancer Research
Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland

兔导管内注射:将目标溶液局部输送到兔乳腺中

Protocol
2,341 Views
03:44 min
April 30, 2023
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Transcript

导管内注射用于通过导管向乳腺提供局部治疗。首先,将一只面朝上腹侧的麻醉兔子放在手术台上进行手术。找到要注射的奶嘴并用酒精擦拭该区域。

在注射器中加入适量的无菌盐水溶液,然后将针头斜面朝上插入奶嘴的一侧。注射生理盐水以更好地观察导管开口。接下来,提起奶嘴并小心地插入钝头针头以插管导管。在注射器中加入要注射的目标溶液。

现在,轻轻地将注射器锁定在针座上,然后缓慢注入溶液,以避免因压力而造成任何损坏。注射到中会将溶液直接输送到兔子乳腺。在以下方案中,我们将使用造影剂在兔模型中使用导管内注射技术可视化乳腺导管。

要开始此过程,请小心地剃掉第三对和第四对腹股沟周围区域的麻醉兔子的尾腹部。去除大部分毛发后,将脱毛霜涂抹在剃光的区域,涂抹后 10 分钟用湿纸巾蘸温水去除霜。然后用浸有酒精的纱布垫擦拭该区域以清洁注射部位。将兔子放在一个 V 形槽中,槽内衬循环温水毯和吸水垫。

要制备造影剂,请根据制造商的说明重新配制,然后轻轻摇动小瓶以混合。这里使用的造影剂在重建后在室温下可稳定保存 4 至 6 小时。找到要注射的第三对和第四对腹股沟奶嘴。然后将 0.2 毫升无菌 0.9% 盐水装入带有 22 号针头的 1 毫升 Luer-lock 结核菌素注射器中。生理盐水进入注射器后,丢弃 22 号针头,并用无菌的 25 号针头更换。

接下来,在纱布垫上用 85% 异丙醇轻轻擦拭该区域。将针头的斜面向上,注射器与动物的身体平行,将针头插入的侧面,缓慢注入 0.1 至 0.2 毫升生理盐水,以便更好地观察导管开口。然后,将 0.2 毫升注射液装入 1 毫升鲁尔锁结核菌素注射器中。

用拇指和食指轻轻握住奶嘴,然后轻轻抬起它以进行导管内注射。在保持的抬起位置的同时,使用 25 号钝尖针头小心地插管感兴趣的导管。插管后,轻轻扭动钝头输液针座上的 Luer-lock 注射器,直至其锁定到位。提起奶嘴并缓慢注入溶液,以尽量减少管道内快速移动的液体造成的潜在损害。

Key Terms and Definitions

  • Intraductal injection - Technique of delivering solutions directly to mammary glands.
  • Rabbit mammary glands - Milk producing glands situated in the teats of rabbits.
  • Rabbit injection sites - Specific places on a rabbit's body where injections are given.
  • Anesthesia for rabbits - Use of drugs to cause loss of sensation or consciousness in rabbits.
  • Localized injection - Injection aimed at a specific part, for a focused therapeutic effect.

Scientific Background

  • Introduce Intraductal Injection - A method for localised delivery of treatments (e.g., intraductal injection).
  • Key Concepts - It involves injecting a solution via the teats (e.g., rabbit injection sites).
  • Underlying Mechanisms - Procedure requires careful handling and proper anesthesia (e.g., anesthesia for rabbits).
  • Connect to Experiment - The knowledge is applied in a procedure involving a contrast agent into a rabbit.

Questions that this video will help you answer

  • What is intraductal injection and how is it used in rabbit mammary glands?
  • Why are specific rabbit injection sites important?
  • How does anesthesia for rabbits play a role in the procedure?

Applications and Relevance

  • Practical Applications - This technique allows for targeted treatments (e.g., localized injection).
  • Industry Impact - Could improve standards in veterinary healthcare (e.g., rabbit anaesthesia).
  • Societal Importance - It contributes to the well-being of pet rabbits (e.g., mastitis rabbit).
  • Link to Scientific Advancements - Propels advances in targeted drug delivery systems.

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