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JoVE Journal
Bioengineering
心肌组织工程生物衍生的注射材料的制备
心肌组织工程生物衍生的注射材料的制备
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

心肌组织工程生物衍生的注射材料的制备

Full Text
15,542 Views
11:32 min
December 20, 2010

DOI: 10.3791/2109-v

Sonya Seif-Naraghi1, Jennifer Singelyn1, Jessica DeQuach1, Pamela Schup-Magoffin1, Karen Christman1

1University of California, San Diego

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Please note that some of the translations on this page are AI generated. Click here for the English version.

准备脱细胞组织注射基质凝胶注入大鼠心肌的方法

由脱细胞、心包和心肌组织产生的基质凝胶保留天然细胞外基质成分,包括各种蛋白质、肽和糖胺聚糖。这些组织基质可用于组织工程。在本视频中,基质凝胶是通过使心包组织脱细胞以产生脱细胞细胞外基质来制造的。

然后处理细胞外基质以产生溶解的液体版本。接下来,在小动物模型中测试溶解的基质,以确定其作为可注射支架的可行性。组织学和免疫组织化学分析表明,在体内,注射的材料会形成多孔纤维支架,允许血管细胞浸润。

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