Whole Mount <em>in Situ</em> Hybridization of E8.5 to E11.5 Mouse Embryos

Qiaozhi Wei; Nancy R. Manley; Brian G. Condie

doi:10.3791/2797

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Biology

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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

全山原位杂交E8.5，E11.5小鼠胚胎

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13:54 min

October 10, 2011

DOI: 10.3791/2797-v

Qiaozhi Wei¹, Nancy R. Manley¹, Brian G. Condie¹

¹Department of Genetics,University of Georgia

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这整个坐骑

该程序的总体目标是定义整个小鼠胚胎中的基因表达模式。这是通过首先设计和生成目标基因的特异性核糖探针来实现的。接下来，收集胚胎并处理杂交。

然后将标记的探针与胚胎杂交。最后一步是通过免疫组织化学检测使杂交探针可视化。最终，可以获得显示整个胚胎或胚胎切片中基因表达模式的结果。

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