收获腹膜脂肪库:一种研究小鼠模型中腹膜脂肪组织卵巢癌定植的技术

脂肪组织或脂肪库主要存在于腹膜的大网膜、肠系膜、子宫、性腺和脾门静脉区域，可能为转移期间的癌细胞定植提供合适的微环境。

要分离这些腹膜脂肪库，首先取一只安乐死的雌性小鼠。通过腹膜壁做一个中线手术切口，露出其内脏器官。现在，找到胃和胰腺脾脏复合体上存在的大网膜脂肪并将其提取出来。切除卵巢周围的性腺脂肪。此外，去除包裹子宫角的子宫脂肪。随后，切开小肠和幽门之间的连接处，释放附着在肠道上的肠系膜脂肪。最后，提取连接脾脏远端和胰腺的脾门脂肪细带。

解剖的脂肪组织保存在合适的冷冻缓冲液中，以保留组织生理机能。分析这些腹膜脂肪组织以评估定植转移癌细胞的存在。

由于采集腹膜脂肪库构成内脏切除，因此通过腹膜壁在腹股沟附近做一个中线切口，以暴露内脏器官。要切除大网膜脂肪，请用镊子将脾脏从腹膜腔中延伸出来，以暴露大网膜胰腺复合体。

对于性腺脂肪，用镊子提起卵巢周围的性腺脂肪，并通过切断组织连接将其切除。立即将组织放入冰冷的PBS中。接下来，使用镊子取出子宫角周围的子宫脂肪，并通过切割组织连接进行切除，然后立即将组织放入冰冷的 PBS 中。

获取肠系膜脂肪时，切开小肠和幽门的交界处。用镊子牢牢抓住小肠的自由端，慢慢将其从肠系膜脂肪上剥离。使用解剖剪刀从肠系膜根部释放肠系膜脂肪。然后，立即将组织放入冰冷的PBS中。

最后，要去除脾门脂肪，请使用镊子抬起脾脏的远端，露出连接脾门和胰腺的细脂肪带。切除脾门脂肪，首先将其从胰腺中释放出来，然后小心地将其从脾脏中解剖。然后，立即将组织放入PBS中。

• Peritoneal Fat - A type of internal organ fat, specifically located within the peritoneal cavity.
• Ovarian Forceps - Surgical tool used to grip and hold ovarian tissue during procedures.
• Omental Fat - Segment of abdominal fat attached to the stomach and draped over the intestines.
• Mesenteric Lift - Technique used to manipulate the mesentery, a membrane connecting intestines to the abdominal wall.
• Gonadal Fat - Fat tissue surrounding the ovaries in females or the testes in males.

• Introducing Peritoneal Fat - The body's form of energy storage, found within the peritoneal cavity (e.g., peritoneal fat).
• Outlining Key Concepts - Grasping the function and structure of different fat deposits (e.g., omental fat).
• Explaining Underlying Mechanisms - Understanding the separation of these fat deposits during surgery or dissection.
• Connecting to Experiment - Determining the biological importance of these different fat deposits.

• What is peritoneal fat and how is it different from omental or gonadal fat?
• What role do tools like ovarian forceps play in surgical procedures?
• What techniques are used to separate distinct fat deposits like mesenteric lift?

• Describing Practical Applications - Utilization of the understanding of different fat deposits in healthcare (e.g., omental fat removal).
• Identifying Industry Impact - Knowledge of these fat deposits aids in surgeries and obesity research (e.g., healthcare).
• Highlighting Societal Importance - Awareness of different body fat types contributes to overall health comprehension (e.g., obesity prevention).
• Linking to Scientific Advancements - Development of more effective and less intrusive surgical techniques.