Extracting Prostate from Mouse Urogenital System: A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse

Encyclopedia of Experiments
Cancer Research

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Encyclopedia of Experiments Cancer Research

从小鼠泌尿生殖系统中提取前列腺:一种将雄性小鼠前列腺与其他泌尿和生殖系统器官分离的技术

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03:24 min

July 8, 2025

Please note that some of the translations on this page are AI generated. Click here for the English version.

泌尿生殖系统（UGS）包括彼此靠近的泌尿系统和生殖系统器官。精囊、前列腺、睾丸和输卵管是生殖系统的一部分，而膀胱和尿道是泌尿系统的一部分。

要分离前列腺，首先将安乐死的雄性小鼠置于手术床上的仰卧位。切开腹部皮肤和肌肉层。继续切过中线以暴露腹腔。

现在，找到充满液体的膀胱。使用镊子将其拉起，然后从鼠标腹部提起整个 UGS。用剪刀剪掉 UGS 下方的组织，直到脊柱，以切断与腹腔的任何连接。将收获的UGS背面朝上放入解剖培养基中，并在显微镜下观察。

接下来，从膀胱底部剪下膀胱，露出下面的前列腺。倒置 UGS 以定位两个管状导管。将它们从底部切下，留下前列腺、精囊和尿道。追踪两个半圆形精囊并在其底部切开它们。由此产生的组织包含包裹在尿道上的前列腺叶。

暴露泌尿生殖系统或UGS后，用中钝镊子牢牢抓住膀胱，并将整个系统从小鼠腹部抬起。将一把剪刀滑到膀胱和前列腺下方，一直到脊柱，在脊髓上做一个切口，然后剪断与腹腔的任何剩余连接，以去除整个 UGS。

在

解剖显微镜下将UGS转移到含有2至6毫升PBS的6厘米培养皿中，并使用一把细镊子和显微解剖剪刀小心地清除组织系统背侧和腹侧的所有脂肪，而不剪断任何前列腺组织。

去除

所有脂肪后，用镊子拉动膀胱并切除膀胱底部的膀胱。将剩余的组织腹侧朝上，用镊子握住一个外导管，用剪刀沿着血管到达其底部，并在其底部切除血管。

移除另一侧的同一血管，并将镊子插入精囊和前列腺之间，以便根据需要剪断任何相邻的结缔组织。然后，将精囊追踪到尿道底部，并在不刺穿血管的情况下取出血管。

Urogenital System - Collective internal organs involved in urinary and reproductive functions.
Prostate Dissection - The process of surgically removing a prostate gland.
Surgical Castration Prostate Cancer - Procedure for removing prostate to treat cancer.
Microdissection Scissors - Small precision scissors used in dissection.
Transfer Forceps - Used for holding and moving tissues during dissection.

Introduce Urogenital System - Organs related to urinary and reproductive functions (e.g., prostate gland).
Key Concepts - Prostate dissection involves careful removal (e.g., microdissection scissors).
Underlying Mechanisms - Critical for treating cases like prostate cancer.
Connect to Experiment - Understanding prostate dissection can inform cancer surgeries.

What is the urogenital system and how to dissect the prostate gland?
How do tools such as microdissection scissors aid in prostate dissection?
What is the connection between prostate dissection and surgical castration for prostate cancer?

Describe Practical Applications - Prostate dissection, crucial in urology procedures (e.g., surgery).
Industry Impact - Broadly impacts healthcare, especially in urology.
Societal Importance - Crucial in treating prostate cancer (e.g., surgical castration).
Link to Scientific Advancements - Informing safer, more effective surgical procedures.