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Neuroscience
硬膜下植入软皮层电图阵列,用于小型猪皮质电生理记录
硬膜下植入软皮层电图阵列,用于小型猪皮质电生理记录
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Encyclopedia of Experiments Neuroscience
Subdural Implantation of a Soft Electrocorticography Array for Cortical Electrophysiology Recording in Minipigs

硬膜下植入软皮层电图阵列,用于小型猪皮质电生理记录

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04:00 min
July 8, 2025
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Please note that some of the translations on this page are AI generated. Click here for the English version.

Transcript

从麻醉的小型猪开始,在听觉皮层上进行双侧开颅手术。

在大脑的外层硬脑膜上开设狭缝。

取一个软皮质电图阵列,由电极条、柔性电缆和带连接器的端口组成。

插入条带,直到电缆边缘位于狭缝顶部,确保电极正确放置在听觉皮层表面上。

在

对侧重复该过程。

在

电缆上放置一个钛桥,固定植入物。

插入地线以建立记录参考。

缝

合硬脑膜,并使用钛桥更换和固定骨瓣。

将

脚踏板和基座连接到头骨上。缝合切口。

让清管器恢复并将无线头台固定在基座上。

该阵列捕获皮质神经元产生的电信号,这些信号从头部级传输到记录系统。

应用声音刺激并记录刺激诱导的听觉神经元活动。

将

植入物放在硬脑膜狭缝上方,并使用小镊子在每个边缘按顺序滑动装置,在硬膜下插入装置。小心地握住设备的基座端,并与植入物一起前进,以免产生阻碍插入的张力。

当连接器边缘位于狭缝顶部时停止插入。要将植入物固定到位,请在开颅手术边缘或锚定翼中将钛桥放在电缆上,并使用适当的螺丝刀用一两个钛螺钉将其固定。

接下来,使用 3-0 可吸收缝合线和小持针器小心地将硬脑膜缝合在种植体电缆周围。尽可能将两个硬脑膜边缘放在一起,不要用缝合线撕开薄膜。

要进行骨瓣放置,请使用钛螺钉在每个骨瓣的前部和后部固定钛桥。将钛桥的末端拧到头骨上。接下来,规划脚踏板的方向。

为确保所有腿都可以拧入头骨,然后拧入脚踏板的钛螺钉,直到其牢固到位,以固定脚踏板。然后,将基座拧到脚踏板上。使用 4-0 不可吸收缝合线创建皮下缝合线,缝合线相距 3 毫米。从基座开始,在切口两侧朝基座移动。接下来,使用 6-0 不可吸收缝合线缝合皮肤,缝合线相距 5 毫米,从而闭合真皮层。

从基座开始,在切口两侧朝基座移动。注意在两个皮瓣之间和基座边缘附近实现良好的组织并置,以避免出现空隙。将无线头台插入动物后,通过抱住动物或通过喂食零食来分散动物的注意力来实现,记录清醒的大脑信号。确保将放大器天线和外部扬声器放置在靠近猪笼的位置,同时记录信号。

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