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JoVE Encyclopedia of Experiments
Neuroscience
使用与 EEG 耦合的平尖探针测量新生儿疼痛诱发的大脑活动
使用与 EEG 耦合的平尖探针测量新生儿疼痛诱发的大脑活动
Encyclopedia of Experiments
Neuroscience
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Encyclopedia of Experiments Neuroscience
Measuring Pain-Evoked Brain Activity in Neonates Using a Flat-Tip Probe Coupled to EEG

使用与 EEG 耦合的平尖探针测量新生儿疼痛诱发的大脑活动

Protocol
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02:50 min
July 8, 2025
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Please note that some of the translations on this page are AI generated. Click here for the English version.

Transcript

从戴着脑电图或脑电图帽的婴儿开始,电极接触头皮。

有源电极位于颅顶,参比电极位于左耳后面,接地电极位于右前额。

水平握住婴儿的右手。

记录背景脑电图以建立基线。

接下来,将一个连接到固定在脑电图记录系统的接触触发装置上的平头探头,对手施加轻微的疼痛刺激。

探头向计算机发送信号,标记刺激触发时间。

刺激激活伤害感受器——皮肤对疼痛敏感的感觉神经元——打开离子通道。

这允许正离子流入,产生动作电位。

这些动作电位通过 Aδ 纤维从伤害感受器传播并投射到脊髓,从那里到达大脑。

有源电极捕获大脑信号,生成识别疼痛反应的脑电图。

首先选择一个新的工作文件。将采样率设置为 2,000 赫兹。将低截止滤波器设置为 1 赫兹的频率。将高截止滤波器设置为 70 赫兹,将陷波滤波器设置为 50 赫兹。选择一个研究名称来存储数据。

开始脑电图和视频录制。当新生儿的右手处于水平位置时,记录背景脑电图活动。手动注释脑电图记录,以记录未施加刺激且婴儿休息的时期。一旦背景脑电图稳定,对新生儿的右手施加所需量的平头探针刺激。

小心垂直于新生儿的手使用平尖探头,以免尖端弯曲并施加正确的力。当平头探头达到皮肤上的标称力时,接触触发装置会产生触发信号。该信号被发送到计算机,用触发标记标记脑电图记录。

施加

刺激后,停止所有记录并记录实验设置细节。取下婴儿头部的电极后,用湿毛巾清洁头皮以去除残留物。

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