-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

CN

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools

Language

zh_CN

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Encyclopedia of Experiments
Neuroscience
相差磁共振成像评估大鼠动脉中的血流
相差磁共振成像评估大鼠动脉中的血流
Encyclopedia of Experiments
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
Encyclopedia of Experiments Neuroscience
Phase Contrast Magnetic Resonance Imaging to Assess Blood Flow in the Arteries of a Rat

相差磁共振成像评估大鼠动脉中的血流

Protocol
302 Views
03:52 min
April 28, 2025
AI Banner

Please note that some of the translations on this page are AI generated. Click here for the English version.

Transcript

取一只麻醉的大鼠,将其固定在磁共振成像或 MRI 系统的床上。

大鼠身上的纱布垫可维持其体温,而身体上的传感器则监测呼吸和心脏活动,确保生理参数稳定。

将老鼠放在扫描仪内。使用相差 MRI,扫描颈部区域以识别颈总动脉或 CCA。

扫描仪检测由血液中质子移动引起的 MR 信号强度波动。

在心动周期的各个阶段,由于周期性收缩和扩张,血流速度随着动脉直径的变化而变化。

垂直于血流方向对齐成像平面以获得动脉的横截面图。

以规定的时间间隔获取图像以计算血流(血流和横截面积的乘积),并评估整个心动周期中血流的变化。

一旦发现动物生理稳定,就开始核磁共振扫描。从 MRI 扫描仪的控制台监视器中选择定位器序列,并使用任何快速图像采集序列(例如快速自旋回波)沿所有三个方向获取侦察图像,以创建冠状、轴向和矢状面图像。

查看侦察图像,确保动物头部和颈部的中心位于磁铁的中心。如有必要,调整动物的位置,直到到达正确的位置。

如果动物被重新定位,请重复侦察图像扫描。一旦大鼠定位正确,从MRI扫描仪的控制台监视器中选择飞行时间血管造影序列,并首先获取2D飞行时间血管造影,以确定颈总动脉的精确解剖位置。确保饱和带处于打开状态并放置在顶部,以避免静脉信号的干扰。

饱和带通常带有飞行时间序列。如果饱和带未显示在显示器上,请通知服务人员。

使用飞行时间血管造影定位颈总动脉后,将相差磁共振成像仪的图像平面瞄准同一动脉的中心,并使其定向,使切片垂直于血流方向。

确保呼吸和心电图门控都连接到 MRI 系统,在监控计算机上显示清晰的信号,并将触发模块设置为从 MRI 扫描仪的控制台监视器以触发模式打开。此时,再次确认动物的生理反应稳定,并验证监控计算机和 MRI 扫描仪的控制台监视器中的门控选择是否都已打开。

请记住确保显示器计算机和显示器 MRI 扫描仪中的一个都已安装。

接下来,从 MRI 扫描仪的控制台监视器中选择 PC MRI 序列的序列并执行门控 PC MRI 扫描。对每个感兴趣的区域重复此过程。对所有区域进行成像后,将动物从扫描仪中取出并将其放回笼子中。

Related Videos

动态皮层活动影像的并发脑电图与功能 MRI 记录及积分分析

11:28

动态皮层活动影像的并发脑电图与功能 MRI 记录及积分分析

Related Videos

11.9K Views

t1 加权 mri 图像中皮质灰质的自动分割

06:48

t1 加权 mri 图像中皮质灰质的自动分割

Related Videos

9.1K Views

功能 MRI 与新型 MRI 兼容手感应机器人设备结合,以评估从手柄缺陷中恢复的个人的康复情况

07:34

功能 MRI 与新型 MRI 兼容手感应机器人设备结合,以评估从手柄缺陷中恢复的个人的康复情况

Related Videos

8.1K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2025 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code