Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging

doi:10.3791/31579

Encyclopedia of Experiments
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

Encyclopedia of Experiments Neuroscience

Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging

使用显微计算机断层扫描成像观察小鼠脑血栓

Protocol

320 Views

02:05 min

July 8, 2025

Please note that some of the translations on this page are AI generated. Click here for the English version.

Transcript

为了通过显微计算机断层扫描对血栓进行成像，在适当的实验时间点，将纤维蛋白靶向的金纳米颗粒稀释至每毫升10毫克10毫摩尔PBS浓度，并将血栓显像剂超声处理30分钟，以确保纳米颗粒的均匀悬浮。

然后，将300微升纤维蛋白靶向颗粒注射到实验动物的静脉中。将动物转移到显微CT成像仪的床上，用一根系在别针上的绳子轻轻拉动上门牙，使颈部伸直。注入金纳米颗粒五分钟后，开始成像。