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Chapters
- 00:05Title
- 01:26Neonatal Cardiomyocyte Isolation
- 03:45Casting Fibrin Gel Constructs via Injection Molding
- 06:56Contraction Force Testing
- 08:17Representative Fibrin Construct Analyses
- 09:15Conclusion
We describe the isolation of neonatal cardiomyocytes and the preparation of the cells for encapsulation in fibrin hydrogel constructs for tissue engineering. We describe methods for analyzing the tissue engineered myocardium after the culture period including active force generated upon electrical stimulation and cell viability and immunohistological staining.
Tags
EncapsulationCardiomyocytesFibrin HydrogelThree Dimensional HydrogelCulture ConditionsSynthetic PolymersPEG-DAPLGANaturally Occurring ProteinsCollagenHyaluronic AcidFibrinPolymerizeMeshWound Healing ProcessesCell-degradableAutologousTemporary ScaffoldIsolation Of Neonatal CardiomyocytesRat PupsPreparation Of CellsEncapsulation In Fibrin Hydrogel ConstructsIn Vitro StudiesFibrinogenThrombinFibrinopeptides FpA And FpB
