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Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
JoVE 杂志
生物学
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JoVE 杂志 生物学
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
DOI:

12:42 min

April 13, 2012

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Chapters

  • 00:05Title
  • 01:20Ethyl Methane Sulfonate (EMS) Treatment
  • 04:25Confocal Screening of M2 and M3 Populations
  • 05:35Mapping
  • 09:10Results: Mutation Mapping using Genechip Arabidopsis ATH1 Genome Array Hybridization and R Software Analysis
  • 12:24Conclusion

Summary

自动翻译

A fundamental quest in cell biology is to define the mechanisms that underlie the identity of the organelles that make eukaryotic cells. Here we propose a method to identify the genes responsible for the morphological and functional integrity of plant organelles using fluorescence microscopy and next-generation sequencing tools.

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