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Chapters
- 00:05Title
- 01:09Culture of hNPCs in a PuraMatrix
- 03:41Immunocytochemical Staining of Entire Matrices
- 05:44Release of hNPCs from the Scaffolds for Flow-cytometry Analysis
- 07:04Quantification of βIII-tubulin Positive Cells by Flow Cytometry
- 08:09Observation and Enumeration of PuraMatrix Encapsulated hNPCs
- 10:37Conclusion
Aqui nós descrevemos o uso de uma auto-montagem 3-dimensional andaime para a cultura humana células progenitoras neurais. Nós apresentamos um protocolo para liberar as células da scaffolds a serem analisados posteriormente por exemplo, por citometria de fluxo. Este protocolo pode ser adaptado para outros tipos de células para realizar estudos detalhados mecanicamente.
Tags
CultivationHuman Neural Progenitor Cells3-dimensionalSelf-assembling Peptide HydrogelGrowthProliferationNeuronal DifferentiationNeurological DisordersNeurodegenerative DiseasesBiomaterial ScaffoldsRegenerative MedicineStem CellsProgenitor CellsCulture Systems3D EnvironmentSurvivalFatePuraMatrixPeptide Hydrogel Scaffold3D-culture SystemNano-fibersXeno-freePM-concentration
