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Chapters
- 00:05Title
- 01:34Egg Preparation
- 02:42Glass Injection Needle Preparation
- 03:25Midbrain Injection
- 04:38Electroporation
- 05:39Incubate and Harvest
- 06:47Results: Embryonic Chick Midbrain In ovo Electroporation Assay
- 08:13Conclusion
To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.
