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JoVE Journal
Neuroscience
线虫跟踪和行为测量
线虫跟踪和行为测量
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
C. elegans Tracking and Behavioral Measurement

线虫跟踪和行为测量

Full Text
19,831 Views
07:36 min
November 17, 2012

DOI: 10.3791/4094-v

Jirapat Likitlersuang1, Greg Stephens2,3, Konstantine Palanski1, William S. Ryu1,4

1Donnelly Centre,University of Toronto, 2Department of Physics and Astronomy,Vrije Universiteit, 3Okinawa Institute of Science and Technology, 4Department of Physics,University of Toronto

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们已经开发出一种视频率跟踪显微镜系统,可以记录和量化

海雅的运动是发现参与海雅行为的基因和神经元的重要工具。Sea Elegance 经历了刻板的运动行为,例如向前和向后运动以及通过改变其形状进行各种类型的转弯。为了以高空间和时间分辨率量化这些长距离运动,我们构建了一个蠕虫跟踪器来捕捉 Sea Elegance 在琼脂平板表面爬行时的运动。

我们还开发了软件,可以使用在 lab view 和 MATLAB 中编写的自定义程序定量地隔离和分析这些运动。所有视频跟踪系统的挑战是它们会生成大量高度维度的数据。我们的图像处理和数据分析程序由于这一挑战,通过将蠕虫形状简化为一组独立的组件,我们将蠕虫行为全面重建为仅 3 到 4 维的函数。

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