Name: 这在朱庇特一月 (视频) | JoVE Journal
Uploaded: 2012-01-02T00:00:00.000+00:00
Duration: 5 min 9 s
Description: 了解神经科学与生物学领域的可视化实验方法。JoVE通过视频协议展示创新技术，帮助研究人员深入理解复杂的生物过程并提高实验的可重复性。

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5 minutes

<h2 class="jove_title"><a href="https://www.jove.com/video/3122">恶性疟原虫寄生虫对人脑内皮细胞细胞粘附</a></h2>的选择
Antoine Claessens， J. Alexandra Rowe 爱丁堡大学免疫、感染和进化中心
描述了一种脑疟疾隔离的体外模型1.选择恶性疟原虫感染的红细胞与永生化的人脑微血管内皮细胞结合。选定的寄生虫显示出独特的表型。可以使用各种恶性疟原虫菌株和内皮细胞系进行选择过程。
<h2 class="jove_title"><a href="https://www.jove.com/video/3308">植入颈动脉袖带触发剪切应力诱导的小鼠动脉粥样硬化</a></h2>
Michael T. 库尔曼1， Simon Cuhlmann2， 3， Irmgard Hoppe1， Rob Krams3， Paul C. Evans2， Gustav J. Strijkers4， Klaas Nicolay4， Sven Hermann1， Michael Schäfers1 1欧洲分子影像研究所，威斯特法伦威廉大学明斯特分校，2英国心脏基金会心血管科学部，伦敦帝国理工学院，3伦敦帝国理工学院生物工程系，4埃因霍温理工大学生物医学工程
本文介绍的收缩袖带旨在诱导小鼠颈总动脉动脉粥样硬化。由于其内腔的圆锥形，植入的袖带产生明确的低、高和振荡剪切应力区域，从而触发不同炎症表型的动脉粥样硬化病变的发展。
<h2 class="jove_title"><a href="https://www.jove.com/video/3330">使用改良的 Zigmond 室测定法分析躯干神经嵴细胞迁移</a></h2>
Christopher C. Walheim1， Juan Pablo Zanin2， Maria Elena de Bellard1 1加州州立大学生物学系，北岭分校，2Centro de Biología Celular y Molecular， Universidad Nacional de Córdoba
描述了一种分析外植细胞（躯干神经嵴细胞）迁移的方法。这种方法便宜、温和，并且能够将趋化性与趋化作用和其他对迁移极性的影响区分开来，例如来自原代主干神经嵴细胞培养物中的细胞间相互作用的影响。
<h2 class="jove_title"><a href="https://www.jove.com/video/3350">胚胎腹侧中脑器官型切片培养物：体外研究多巴胺能神经元发育的系统</a></h2>
Gabriela Oana Bodea， Sandra Blaess 波恩大学神经重建研究所
描述了一种从 E12.5 小鼠胚胎中脑生成器官型切片的方法。器官型切片培养物可用于观察多巴胺能神经元或其他腹侧中脑神经元的行为。
<h2 class="jove_title"><a href="https://www.jove.com/video/3362">使用亲脂性 Vital 染料 DiI 对秀丽隐杆线虫角质层结构进行可视化</a></h2>
Robbie D. 舒尔茨1， 2， <字体 color='黑色'>蒂娜 L. Gumienny2 1德克萨斯 A&M 大学系统健康科学中心分子和细胞医学系，2德克萨斯 A&M 大学系统健康科学中心医学院分子和细胞医学系
我们提出了一种使用红色荧光亲脂染料 DiI（1,1'-二十八烷基-3,3'，3'-四甲基吲哚羰花青高氯酸盐）在活秀丽隐杆线虫中可视化角质层的方法，该染料通常用于秀丽隐杆线虫中，以可视化环境暴露的神经元。通过这种优化的方案，鼻翼和环形表皮结构通过 DiI 染色并使用化合物显微镜观察。
<h2 class="jove_title"><a href="https://www.jove.com/video/3406">一种检测和清除主流卷烟烟雾中气相自由基的协议</a></h2>
长习 Yu1， Boris G. Dzikovski2， Jack H. 释放3， 2 1CDCF-AOX 实验室， 2康奈尔大学化学与化学生物学系国家先进 ESR 技术生物医学中心 （ACERT）， 3康奈尔大学贝克实验室化学与化学生物学中心
自旋捕获 ESR 光谱研究植物抗氧化剂番茄红素、碧萝芷和葡萄籽提取物对清除卷烟烟雾中气相自由基的影响。
<h2 class="jove_title"><a href="https://www.jove.com/video/3417">如何从 MR 图像中测量皮质折叠：计算局部 Gyrification 指数的分步教程</a></h2>
Marie Schaer1， Meritxell Bach Cuadra2， 3， Nick Schmansky4， Bruce Fischl4， Jean-Philippe Thiran2， Stephan Eliez1 1日内瓦大学精神病学系医学， 2洛桑联邦理工学院信号处理实验室， 3洛桑大学医院中心和大学放射科， 4麻省总医院 Athinoula A. Martinos 生物医学成像中心
在任何年龄测量回旋（皮质折叠）都代表着大脑早期发育的窗口。因此，我们之前开发了一种算法来测量半球1 上数千个点的局部回转。在本文中，我们详细介绍了该局部回转指数的计算。
<h2 class="jove_title"><a href="https://www.jove.com/video/3551">使用红外热成像检测迟发性肌肉酸痛</a></h2>
Hani H. Al-Nakhli1， Jerrold S. 彼得罗夫斯基1， 2， 迈克尔 S. Laymon2， 李 S. Berk1 1Loma Linda University， 2Azusa Pacific University
这项调查的目的是评估使用红外热像仪是否是检测和量化运动后肌肉酸痛的有效工具。

january 2012: this month in jove

January 2012: This Month in JoVE

The Journal of Visualized Experiments (JoVE) begins 2012 by publishing its 1500th article, which focuses on a method for analyzing the free radical composition of cigarette smoke. Although tobacco tar is thought to be the most carcinogenic agent in cigarette smoke, free radicals are well known cancer-causing molecules, and the level of these carcinogens contained in cigarette smoke is not currently known. The method employed in this article combines the use of a single-port smoking device, which simulates cigarette puffing, with electron spin resonance (ESR) spectroscopy, a technique, which measures the spin energy emitted by the unpaired electrons in free radicals. One important modification of the single port smoking device used in this article, is the introduction of a liquid nitrogen trap in front of the spin trap. Liquid nitrogen removes water vapor from cigarette smoke and greatly improves ESR spectra as a result.
In addition to measuring the free radical concentration found in cigarette smoke, plant antioxidants are introduced into modified cigarette filters and the ESR spectra of cigarette smoke passed through these filters is obtained. Grape seed extract and lycopene were observed to have the most significant scavenging effects on free radicals, and if cost effective means of including these molecules into cigarette filters can be developed, a less harmful cigarette could be created.
In Clinical and Translational Medicine, JoVE presents an analytical method for determining the local Gyrification Index (lGI) - a measurement of the extent of cortical folding. Cortical folds develop during the first few post natal months, and adverse events during this time can lead to abnormalities in the brain's surface anatomy, which may ultimately correlate with neuropsychiatric disorders.
The analysis tool developed for calculating the local Gyrification Index is Freesurfer, which can convert T1 weighted MRI scans into 3D reconstructions of the cortex. Separate reconstructions are created for the grey/white mater and grey/pial interfaces, which are manually adjusted before computating the lGI. Following a smoothing operation, circular regions of the pial and grey/white surfaces are compared, in order to compute the lGI for thousands of points over the entire brain. Freesurfer makes analysis of gyrification straightforward and accessible to clinicians and brain scientists, who are interested in comparing the extent of cortical folding between different individuals in normal and disease states.
JoVE's Immunology and Infection article for the month of January continues the journal's tradition of publishing cutting edge methodology in vector born disease research. Specifically, this article adds to JoVE's comprehensive collection of protocols for malaria research by extending our library to cerebral malaria - a devastating condition with high susceptibility in children. Briefly, this protocol involves exposing a monolayer of human brain endothelial cells, passaged onto petri dishes, to Plasmodium falciparum-infected red blood cells. Following washing and enriching steps, high yields of parasites, which carry the potential to infect the brain, can be isolated and further characterized.
In Neuroscience, JoVE showcases a method for studying the migration of neural crest cells. These cells are a transient, multipotent, developmental cell type that can, in addition to becoming neuronal tissue, differentiate into smooth muscle, chromaffin cells, cartiliage, and bone. Chick embryos are carefully prepared, so that the trunk region can be isolated and the neural tube excised for overnight culture on fibronectin-coated coverslips, which allows neural crest cells to emigrate from the neural tube. Following removal of the neural tube, coverslips containing the cultured neural crest cells are transferred to a modified Zigmond chamber - a chamber which enables a concentration gradient of putative chemotactic agent to be established across the culture. Timelapes imaging followed by analysis of cell trajectories, is used to assess the chemotactic potential of molecules loaded into the zigmund chamber, providing insight into the molecular cues that guide migration of this important developmental cell type.
These featured articles comprise four of fifty video-protocols for release in January. Other notable articles include methods for visualizing the cuticle structures of c. elegans, thermal imaging of delayed onset muscle soreness, culturing the ventral midbrain in organotypic slices, and inducing atherosclerosis in mice.

Here are some highlights from the January 2012 Issue of Journal of Visualized Experiments (JoVE).

The Journal of Visualized Experiments (JoVE) begins 2012 by publishing its 1500th article, which focuses on a method for analyzing the free radical ...

Research

科研

JoVE Journal

Biology

生物学

这在朱庇特一月

了解神经科学与生物学领域的可视化实验方法。JoVE通过视频协议展示创新技术，帮助研究人员深入理解复杂的生物过程并提高实验的可重复性。

这在朱庇特一月 (视频) | JoVE Journal

Aaron Kolski-Andreaco

January 2012: This Month in JoVE

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