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Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
JoVE 杂志
生物学
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JoVE 杂志 生物学
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

11,258 Views

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07:19 min

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September 28, 2012

DOI:

10.3791/4350-v

DOI:
10.3791/4350-v

•

07:19 min
September 28, 2012

•

3 Views
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1Max Delbrück Center for Molecular Medicine

Summary

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Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.

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Cite this Article

Lombardo, V. A., Sporbert, A., Abdelilah-Seyfried, S. Cell Tracking Using Photoconvertible Proteins During Zebrafish Development. J. Vis. Exp. (67), e4350, doi:10.3791/4350 (2012).

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