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Neuroscience
斑马鱼幼虫的前脑的电生理记录
斑马鱼幼虫的前脑的电生理记录
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Forebrain Electrophysiological Recording in Larval Zebrafish

斑马鱼幼虫的前脑的电生理记录

Full Text
17,784 Views
06:00 min
January 24, 2013

DOI: 10.3791/50104-v

Scott C. Baraban1

1Epilepsy Research Laboratory, Department of Neurological Surgery,University of California, San Francisco

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Please note that some of the translations on this page are AI generated. Click here for the English version.

外场电位记录在幼虫斑马鱼的前脑的一个简单的方法来描述。该方法提供了一个强大的

该程序的总体目标是从完整幼虫斑马鱼的前脑获得细胞外场记录。这是通过首先将斑马鱼幼虫固定在 aros 块中来实现的。接下来,在直接可视化下将记录微电极放置在前脑中。

最后一步是记录前脑电活动。最终,结果可用于监测完整斑马鱼幼虫的异常电图癫痫发作活动。当我试图证明斑马鱼幼虫可以引起癫痫发作时,我第一次想到了这种方法 斑马鱼养殖遵循前面描述的标准程序。简要。

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