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使用自制双辉光萤光素酶检测的高通量筛选功能

JoVE 杂志
生物学

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JoVE 杂志生物学

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

使用自制双辉光萤光素酶检测的高通量筛选功能

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23,382 Views

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12:55 min

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June 01, 2014

DOI:

10.3791/50282-v

DOI:

10.3791/50282-v

•

12:55 min

June 01, 2014

•

14 Views

Jessica M. Baker¹, Frederick M. Boyce¹

¹Department of Neurology,Massachusetts General Hospital

Summary

我们目前确定采用高通量机器人转染和一个自制的双发光荧光素酶检测转录调控因子的快速和廉价的检查方法。该协议迅速产生直接并排端功能数据数以千计的基因，很容易修改针对任何感兴趣的基因。

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