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Microfluidic Chip Fabrication and Method to Detect Influenza
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Bioengineering
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JoVE Journal Bioengineering
Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

DOI:
10.3791/50325-v

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09:43 min

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March 26, 2013

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, ,
1Department of Mechanical Engineering,Boston University , 2Department of Biomedical Engineering,Boston University

Chapters

  • 00:05Title
  • 01:10Chip Fabrication
  • 04:33Solid Phase Extraction Method
  • 06:43RT-PCR
  • 08:06PCR Products Detection
  • 08:38Results: The Microfluidic Assay Flow
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

An integrated microfluidic thermoplastic chip has been developed for use as a molecular diagnostic. The chip performs nucleic acid extraction, reverse transcriptase, and PCR. Methods for fabricating and running the chip are described.

Tags

Keywords: Microfluidic ChipInfluenza DetectionDiagnosticsRT-PCRSample PreparationLab-on-a-chipVirus DetectionPathogen DetectionHigh-throughput Manufacturing

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