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Immunology and Infection
分离恶性疟原虫晚期感染的红细胞由磁性装置
分离恶性疟原虫晚期感染的红细胞由磁性装置
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

分离恶性疟原虫晚期感染的红细胞由磁性装置

Full Text
14,443 Views
05:20 min
March 2, 2013

DOI: 10.3791/50342-v

Lorena Michelle Coronado1,2, Nicole Michelle Tayler1,2, Ricardo Correa1,2, Rita Marissa Giovani3, Carmenza Spadafora1

1Centro de Biología Celular y Molecular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)

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Please note that some of the translations on this page are AI generated. Click here for the English version.

顺磁性质的疟原虫色素是用来隔离后期阶段

恶性疟原虫是一种人类寄生虫,仅生活在红细胞内。在生长过程中,它利用红细胞内的分子以它们为食,由于血红蛋白中的铁分子对寄生虫有毒,因此它会将它们放入称为血红素的惰性晶体中。这种血液晶体赋予寄生虫一些超磁性。

正是这种顺磁性用于将受感染的红细胞与内部不携带寄生虫的红细胞分开。通过使用磁铁,可以浓缩寄生虫百分比非常低的培养物,以实现近 100% 的寄生虫。在许多实验中,感染者与未感染的红细胞的这种分离是非常可取的,在这些实验中,需要在没有剩余红细胞的混杂因素的情况下研究寄生虫的特定特征。

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感染 第73 传染病学 分子生物学 细胞生物学 免疫学 医药学 寄生虫学 恶性疟原虫细胞培养技术 疟原虫色素 磁珠 裂殖体的净化 顺磁性 红细胞 红细胞 疟疾原虫 寄生虫 隔离 细胞培养

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