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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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神经科学
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JoVE 杂志 神经科学
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

DOI:
10.3791/50412-v

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11:48 min

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July 24, 2013

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, , ,
1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Chapters

  • 00:05Title
  • 02:06Preparation of DNA and Glass Micropipettes for Injection
  • 02:57Laparotomy
  • 04:33DNA Injection into the Brain Ventricle
  • 05:59Targeting the Hypothalamus for Electroporation
  • 07:17Finishing the Surgery
  • 08:28Analyzing the Results
  • 09:18Results: Hypothalamus Transfection by In utero Electroporation
  • 10:54Conclusion

Summary

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Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

Tags

Genetic ManipulationMouseDeveloping HypothalamusIn Utero ElectroporationGenetic ModificationMammalian BrainMouse MutantsDeveloping BrainCortexThalamusHypothalamusHypothalamic NeuroepitheliumMammillary BodyElectroporation

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