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Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

Article DOI: 10.3791/50417-v • 08:00 min • April 22nd, 2014
April 22nd, 2014 •
1,2,3, 1,4
1Molecular Neurobiology, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 4College of Optometry, Western University of Health Sciences

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Imaging of cerebrovascular development in larval zebrafish is described. Techniques to facilitate 3D imaging and modify cerebrovascular development using chemical treatments are also provided.

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Imaging 3D Reconstruction Cerebrovascular Structures Embryonic Zebrafish Developmental Biology Pathology Visual Examination Zebrafish Embryos Heart Development Vascular Development Transgenic Zebrafish EGFP Expression Vascular Endothelial Cells Confocal Microscopy Protocols Fixation Protocols EGFP Fluorescence Preservation High-quality Images Cerebrovascular Structures Imaging Neural Tissue Preservation Confocal Imaging Pitfalls 3D Reconstructions Open Source Software
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