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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Article DOI: 10.3791/50434-v • 10:02 min • June 27th, 2013
June 27th, 2013 •
1,2, 1,2
1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School

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We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

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Xenopus Tropicalis Egg Extracts Microtubule-associated RNAs Transcriptome Localization Subcellular Destinations Gene Expression Control Biochemically Purify High-throughput Sequencing Technologies Fully Sequenced Genome X. Laevis X. Tropicalis Egg Extracts Microtubule Polymerization Isolation Subcellular Structures Localized RNAs
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