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Biochemical Titration of Glycogen In vitro
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JoVE Journal Biology
Biochemical Titration of Glycogen In vitro

Biochemical Titration of Glycogen In vitro

DOI:
10.3791/50465-v

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07:16 min

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November 24, 2013

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, , ,
1Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 – INSERM, U1081 – UNS, Centre Antoine Lacassagne,University of Nice – Sophia Antipolis

Chapters

  • 00:05Title
  • 00:54Cell Lysis
  • 02:50Hydrolysis of Glycogen
  • 03:55Development and Reading of Fluorescence
  • 05:28Results: Glycogen Quantification and Its Role in Hypoxia
  • 06:49Conclusion

Summary

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We describe here an accurate, reproducible and convenient biochemical method for titration of glycogen in vitro. This technique uses the Abcam Glycogen assay kit and is based on successive hydrolysis of glycogen to glucose and glucose titration by fluorescence.

Tags

GlycogenBiochemical TitrationIn VitroGlucoseGlucoamylaseOxiRed ProbeFluorescenceCell LineCCL39LS174NormoxiaHypoxia

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