Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D <sup>1</sup>H NMR

Michal S. Shoshan; Edit Y. Tshuva; Deborah E. Shalev

doi:10.3791/50747

Journal

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化学

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D <sup>1</sup>H NMR

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

9,529 Views

•

14:44 min

•

December 16, 2013

DOI:

10.3791/50747-v

DOI:

10.3791/50747-v

•

14:44 min

December 16, 2013

•

10 Views

Michal S. Shoshan¹, Edit Y. Tshuva¹, Deborah E. Shalev²

¹Department of Chemistry,The Hebrew University of Jerusalem, ²Wolfson Centre for Applied Structural Biology,The Hebrew University of Jerusalem

Summary

The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

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Peptide-metal Complexes Copper (I) Binding Metallochaperone Transport Proteins NMR Spectroscopy 1D And 2D 1H NMR Structure Determination Molecular Mechanics Calculations Solution Structure Unbiased Structure Determination

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR