Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Reema P. Vazirani; Xavier Fioramonti; Vanessa H. Routh

doi:10.3791/50861

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

膜下丘脑腹内侧神经元的电位染料成像从成年小鼠为研究葡萄糖传感

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11:10 min

November 27, 2013

DOI: 10.3791/50861-v

Reema P. Vazirani¹, Xavier Fioramonti², Vanessa H. Routh¹

¹Department of Pharmacology and Physiology,Rutgers New Jersey Medical School, ²Center for Taste and Feeding Behavior,Universite de Bourgogne

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从成年鼠龄的单神经元的活性可以通过从特定脑区域的神经元解离，并使用荧光膜电位染料成像进行研究。通过测试响应变化的葡萄糖，这种技术可以用来研究成年腹内侧下丘脑神经元的葡萄糖敏感性。

该程序的总体目标是使用膜电位敏感染料作为神经元活动的指标，评估老年小鼠腹侧内侧下丘脑或 VMH 神经元的葡萄糖感应。这是通过在切除大脑之前首先进行心脏灌注以将血液从麻醉小鼠的大脑中冲洗出来来实现的。第二步是创建冠状脑切片并解剖 VMH。

接下来，将 VMH 神经元解离并在盖玻片上培养。最后一步是测量神经元的荧光，因为在膜电位染料存在下葡萄糖会降低。最终，每个神经元的荧光变化用于量化葡萄糖抑制神经元对葡萄糖变化的反应。

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神经科学 81期膜电位染料丘脑腹内侧成人的神经元葡萄糖传感荧光成像弓状核