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DOI: 10.3791/50880-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
从成人的大脑收获的神经干细胞被利用,应用范围从神经系统发育的基础研究,以探讨在再生医学潜在的临床应用越来越多。这使得严格控制用于种植这些细胞对于合理的实验结果的分离和培养条件。
以下实验的总体目标是从成年啮齿动物大脑的不同区域生成神经干细胞培养物。这是通过从感兴趣区域收集组织来检查神经干细胞的存在和潜力来实现的。作为第二步,将组织加工成细胞悬液,作为单层培养物进行铺板。
接下来,使用确定的培养条件选择性地增殖神经干细胞,验证其自我更新潜力。体外结果表明,神经干细胞是多效的,这取决于它们的分化潜力,以及各种因素如何影响它们的自我更新和命运。这种方法可以帮助回答神经科学和再生医学领域的关键问题,例如内源性神经干细胞的位置以及如何作它们。
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