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Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction
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生物工程
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JoVE 杂志 生物工程
Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

DOI:
10.3791/50918-v

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10:37 min

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January 16, 2014

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,
1Department of Biomedical Engineering,University of Iowa

Chapters

  • 00:05Title
  • 01:42Fibrin Gel Preparation
  • 04:41Cell Explant Preparation
  • 07:42Time-lapse Imaging and Strain Tracking
  • 09:23Results: Time-lapse Microscopy and Strain Tracking of Fibrin Reorganization
  • 09:58Conclusion

Summary

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Time-lapse microscopy and image processing techniques were used to observe and analyze fibroblast-mediated gel compaction and fibrin fiber realignment in an environmentally controlled bioreactor over a 48 hr period.

Tags

Fibrin Gel CompactionFibroblast-mediated ContractionGel Microstructure ReorganizationTime-lapse DIC MicroscopyStrain MappingMechanobiologyCell-matrix InteractionsWound Healing

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