Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Christina Stoeckle; Ioanna A. Rota; Eva Tolosa; Christoph Haller; Arthur Melms; Eleni Adamopoulou

doi:10.3791/50951

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

从人类胸腺髓样树突状细胞和上皮细胞的分离

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09:07 min

September 19, 2013

DOI: 10.3791/50951-v

Christina Stoeckle^1,2, Ioanna A. Rota¹, Eva Tolosa³, Christoph Haller⁴, Arthur Melms⁵, Eleni Adamopoulou¹

¹Department of General Neurology,Hertie Institute for Clinical Brain Research, ²Institute of Pharmacology,University of Bern, ³Department of Immunology,University Medical Center Hamburg-Eppendorf, ⁴Department of Thoracic and Cardiovascular Surgery,University Clinic Tuebingen, ⁵Department of Neurology,University Hospital Erlangen

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这个协议的细节的方法来分离抗原通过不同的步骤的组织随后在单细胞悬浮液的密度离心和感兴趣的细胞群的最后磁和/或FACS分选的酶消化从人胸腺呈递细胞。

该程序的总体目标是从人胸腺中分离树突状细胞和上皮细胞。这是通过首先通过机械破碎来粉碎解剖组织来实现的。接下来，组织片段进一步酶消化以产生单细胞悬液。

最后，通过 perca 分离富集细胞悬液以去除细胞的低密度部分。最终，树突状细胞和上皮细胞可以通过磁性或事实分选或两者的组合从该组分中分离出来。我们在这里介绍的方法为成功分离和随后研究稀有细胞群（如人胸腺中发现的树突状细胞和胸腺上皮细胞）提供了一种高效、可重现且具有成本效益的程序。

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