Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Stefano G. Daniele; Amanda A. Edwards; Kathleen A. Maguire-Zeiss

doi:10.3791/51005

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠

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09:12 min

January 30, 2014

DOI: 10.3791/51005-v

Stefano G. Daniele¹, Amanda A. Edwards¹, Kathleen A. Maguire-Zeiss¹

¹Department of Neuroscience,Georgetown University Medical Center

Please note that some of the translations on this page are AI generated. Click here for the English version.

一键小胶质细胞生物学的研究成功是小胶质细胞免疫功能的体外从中枢神经系统组织分离过程中的保存。通过旋转振动造成的高纯度和immunofunctional细胞培养物中分离的小胶质细胞所评估的荧光成像，免疫细胞化学，ELISA和以下的小胶质细胞活化的促炎性刺激脂多糖（LPS）和Pam ₃ CSK _{4（PAM）。}

该程序的总体目标是从小鼠新生儿中分离皮质小胶质细胞。这是通过首先从 Muren 新生儿解剖皮质脑组织来实现的。第二步是在体外培养混合神经胶质细胞培养物 17 至 21 天。

接下来，从这些混合神经胶质细胞培养物中分离出小胶质细胞。最后一步是将小胶质细胞接种在细胞培养板中以进行进一步实验。最终，免疫荧光显微镜和 Eliza 用于表明该分离方案保留了小胶质细胞免疫表型和功能。

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