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DOI: 10.3791/51041-v
Hui-Wen Cheng*1,2, Sudeshna Fisch*1, Susan Cheng1, Michael Bauer1, Soeun Ngoy1, Yiling Qiu1, Jian Guan1, Shikha Mishra1, Christopher Mbah1, Ronglih Liao1
1Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital,Harvard Medical School, 2Cardiovascular Department,Chang Gung Memorial Hospital
Please note that some of the translations on this page are AI generated. Click here for the English version.
右心室(RV)的功能障碍是心血管疾病的发病机制中的关键的,但有限的方法可用于它的评估。在超声成像的最新进展提供了一种无创,准确的选择纵向房车研究。在此,我们用详细的RV压力超负荷小鼠模型一步一步的超声心动图的方法。
该程序的总体目标是建立一种非侵入性方法来评估肺动脉缩窄小鼠模型中右心室的结构和功能。这是通过首先通过超声获得胸骨旁长轴 B 和 M 模式视图来实现的,以获得右心室腔尺寸缩短分数和右心室壁厚度。第二步是获得中层的胸骨旁短轴视图,以计算分数面积变化。
接下来,获得主动脉瓣水平的胸骨旁短轴视图,以获得 RV 壁厚和肺动脉峰值速度。最后一步是获得 RV 和 PA 的改良胸骨旁长轴视图 为了计算肺动脉峰值速度 PA 可以从短轴和长轴进行计算。最终,基于上述图像,第 5 步,可以离线进行数据计算和分析。
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