Investigating the Effects of Probiotics on Pneumococcal Colonization Using an <em>In Vitro</em> Adherence Assay

Eileen M. Dunne; Zheng Q. Toh; Mary John; Jayne Manning; Catherine Satzke; Paul Licciardi

doi:10.3791/51069

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

使用一个调查益生素对肺炎球菌定植的影响体外粘附试验

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09:20 min

April 28, 2014

DOI: 10.3791/51069-v

Eileen M. Dunne¹, Zheng Q. Toh², Mary John³, Jayne Manning^1,4, Catherine Satzke*^1,4, Paul Licciardi*²

¹Pneumococcal Research,Murdoch Childrens Research Institute, ²Allergy & Immune Disorders,Murdoch Childrens Research Institute, ³Department of Otolaryngology,The University of Melbourne, ⁴Department of Microbiology & Immunology at the Peter Doherty Institute for Infection & Immunity,The University of Melbourne

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体外坚持测定可用于研究肺炎链球菌的附着上皮细胞单层，并探讨潜在的干预措施，如使用益生菌抑制肺炎球菌定植。

该程序的总体目标是检查益生菌链球菌细胞 levarius 抑制肺炎球菌对上皮细胞系的粘附的能力。这是通过首先制备 CCL 23 上皮细胞单层来实现的。在 24 孔板中，接下来将 slovar 和肺炎球菌依次加入单层并孵育细胞。

在最后一步中，清洗孔以去除非粘附细菌。裂解 CCL 23 上皮细胞并收获孔内容物。最终，可以通过量化细胞相关肺炎球菌的数量来评估唾液对肺炎球菌依从性的影响。

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免疫学第86期革兰氏阳性菌感染肺炎细菌性肺疾病呼吸道感染肺炎链球菌黏附定植益生菌唾液链球菌体外分析