Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Lorena Stannek; Richard Egelkamp; Katrin Gunka; Fabian M. Commichau

doi:10.3791/51196

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Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

通过共生荧光标记菌株监测细菌细胞群中的物种内部竞争

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06:45 min

January 18, 2014

DOI: 10.3791/51196-v

Lorena Stannek¹, Richard Egelkamp¹, Katrin Gunka¹, Fabian M. Commichau¹

¹Department of General Microbiology,Georg-August University

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细菌在有生之年可能会积累有害或有益的突变。在积累有益突变的细胞群中，个体可能迅速比其研究员竞争。在这里，我们介绍了一个简单的程序，以可视化物种内部竞争的细菌细胞种群随着时间的推移使用荧光标记的个人。

该方法使用荧光标记的 bacillus sutilis 的共培养来监测细菌细胞群中随时间推移的快速克隆扩增和有益和有害突变的消除。首先，为竞争实验准备细菌的预培养物。混合等量的荧光标记菌株，并在不同的营养条件下共培养细菌。

然后在不同的时间点收集样品，适当稀释并在琼脂平板上繁殖细胞。现在通过立体荧光显微镜观察和计数幸存的菌株。最终，结果可以确定存活细菌相对于计数菌落总数的百分比。

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细胞生物学第83期杆菌亚蒂利斯进化适应选择性压力有益突变物种间竞争荧光标签荧光显微镜