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Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
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Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

DOI:
10.3791/51302-v

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11:11 min

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June 10, 2014

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, , ,
1Department of Physics,University of Massachusetts Boston

Chapters

  • 00:05Title
  • 01:25Culturing Tumor Spheroids
  • 02:54Preparing 3D Tumor Spheroids Embedded in ECM-containing Tracer Probes
  • 05:33Construction of Sample Point Grid and Video Acquisition at Each Point
  • 07:34Video Data Analysis for Computation of Rheological Properties at Each Spatial Position
  • 09:10Results: Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models
  • 10:26Conclusion

Summary

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Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.

Tags

3D Tumor ModelExtracellular MatrixParticle-tracking MicrorheologyViscoelastic Shear ModulusMechanosensitive InteractionsMatrix RemodelingPancreatic Cancer

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