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内皮细胞小管形成实验的<em>在体外</em>研究血管生成

JoVE 杂志
生物学

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JoVE 杂志生物学

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

内皮细胞小管形成实验的<em>在体外</em>研究血管生成

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59,312 Views

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08:12 min

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September 01, 2014

DOI:

10.3791/51312-v

DOI:

10.3791/51312-v

•

08:12 min

September 01, 2014

•

78 Views

Katie L. DeCicco-Skinner¹, Gervaise H. Henry¹, Christophe Cataisson², Tracy Tabib¹, J. Curtis Gwilliam¹, Nicholas J. Watson¹, Erica M. Bullwinkle¹, Lauren Falkenburg¹, Rebecca C. O’Neill¹, Adam Morin¹, Jonathan S. Wiest²

¹Department of Biology,American University, ²Laboratory of Cancer Biology and Genetics,National Cancer Institute, NIH

Summary

小管形成实验是测量在体外血管生成一种快速的，可量化的方法。内皮细胞结合的条件培养基并接种于基底膜提取物。管形成时小时内新形成的小管很容易量化。

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