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Preparation of Neuronal Co-cultures with Single Cell Precision
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神经科学
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JoVE 杂志 神经科学
Preparation of Neuronal Co-cultures with Single Cell Precision

Preparation of Neuronal Co-cultures with Single Cell Precision

DOI:
10.3791/51389-v

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09:06 min

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May 20, 2014

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, , , , ,
1Leibniz-Institut für Analytische Wissenschaften,ISAS, 2Department of Biochemical Engineering,University College London, 3Institute for Life Sciences,University of Southampton

Chapters

  • 00:05Title
  • 02:04Microfluidic Device Replication in PDMS
  • 03:26Device Assembly
  • 04:40Water Masking for In situ Biomaterial Patterning by Plasma Stenciling
  • 06:39Microfluidic Single Neuron Arraying and Culture
  • 07:49Selective Fluidic Treatment
  • 08:37Conclusion

Summary

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Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs.

Tags

MicrofluidicCo-cultureSingle CellCampenot Chamber神经科学NeurobiologyLow Abundance CellsPrimary DopaminergicSubstantia NigraSpiral GangliaDrosophilia MelanogasterHigh ThroughputCell PatterningPlasma PatterningBiomaterial CoatingsOutgrowthConnectivity

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