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The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
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JoVE 杂志 免疫与感染
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

DOI:
10.3791/51391-v

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12:27 min

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April 02, 2014

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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Chapters

  • 00:05Title
  • 01:24Serum and Bronchoalveolar Lavage Fluid (BALF) Collection
  • 05:27Histopathology Preparation
  • 07:52Results: Representative Immuno- and Histopathological Analyses of LPS-treated Lung Tissue
  • 12:00Conclusion

Summary

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The host immune response to pathogen infection is a tightly regulated process. Utilizing a lipopolysaccharide lung exposure model in mice, it is possible to conduct high resolution evaluations of the complex mechanisms associated with disease pathogenesis.

Tags

Oropharyngeal Intratracheal AdministrationBronchoalveolar LavageHost Immune ResponseLipopolysaccharidePAMPAcute Lung InflammationInnate ImmunityHost-pathogen InteractionMouse Model

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