标记的细胞核从亲和力为基础的隔离<em>果蝇</em>组织的基因表达分析
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12:48 min
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March 25, 2014
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果蝇组织中往往含有细胞类型的非均相混合物。为了检验从一个特定的组织特异性细胞类型的基因表达，细胞核可遗传标记，随后使用具有亲和力为基础的方法分离出来。分离的细胞核，可用于下游应用，如基因表达分析和染色质免疫沉淀。
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Cite this Article
Ma, J., Weake, V. M. Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis. J. Vis. Exp. (85), e51418, doi:10.3791/51418 (2014).
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